Minimal residual disease: the advantages of digital over analog polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Detection of minimal residual disease in acute leukemia by immunological marker analysis and polymerase chain reaction.
Detection of minimal residual disease (MRD) can be useful for adaptation or stratification of treatment in acute leukemia patients and may finally result in individualization of treatment protocols. Although leukemic cells generally have immunophenotypes comparable to their normal counterparts, it is possible to use immunological marker analysis for the detection of MRD based on the assumption ...
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New therapeutic strategies developed recently for chronic lymphocytic leukemia (CLL) have led to remarkable treatment response rates and complete hematological remissions. This means highly sensitive and specific techniques are increasingly needed to evaluate minimal residual disease (MRD) in CLL patients. Quantitative MRD levels can be used as prognostic markers, where total MRD eradication is...
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In acute lymphoblastic leukemia (ALL), remission is classically defined as the reestablishment of normal hematopoiesis and the presence of less than 5% of the nucleated blast cell population found by conventional microscopy; this is used in older protocols to assess treatment response. Morphological analysis, although useful and applicable at any center, has proven to be of limited sensitivity,...
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The Philadelphia (Ph1) chromosome in chronic myelogenous leukemia (CML) involves reciprocal translocation of the bcr gene and the c-abl oncogene. The fused bcr/abl gene is transcribed into two types of chimeric mRNA. By means of a combined method of S1 nuclease protection and polymerase chain reaction, we amplified sequences representative of the chimeric bcr/abl transcripts. Only 5 micrograms ...
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ژورنال
عنوان ژورنال: Leukemia & Lymphoma
سال: 2011
ISSN: 1042-8194,1029-2403
DOI: 10.3109/10428194.2011.580481